Process for mounting tissue sections with an U.V. light curable

Details Process for mounting tissue sections with an U.V. light curable Process for mounting tissue sections with an U.V. light curable

1976-12-10

1978-10-17

Silverberg, Sam

Coating processes

Medical or dental purpose product; parts; subcombinations;...

Particulate or unit-dosage-article base

156 57, 424 3, G01N 128

Patent

active

041209915

ABSTRACT:
Apparatus and method for permanently and protectively mounting "wet" thin sections of biological specimens on microscope slides, wherein each specimen is initially bathed in a low-volatile solvent containing a low concentration of first polymerizable material. Such solvent is allowed to evaporate, whereby the liquid polymerizable material permeates and protects the specimen. Following solvent evaporation, a second polymerizable material is layered over the specimen. Preferably, the first and second polymerizable materials comprise a mixture of low-volatility, low-viscosity, liquid acrylic reactomers and a U.V. light-sensitive catalyst system. A conventional cover slip or other planar transparent member can be positioned over the polymerizable materials, and the same are polymerized by exposure to U.V. radiation. The first and second now-polymerized materials encapsulate the specimen on the microscope slide, become integral and completely hardened and are fully devoid of any solvent. The microscope slide is immediately available for examination.

REFERENCES:
patent: 3466209 (1969-09-01), Leveskis
patent: 3467617 (1969-09-01), Weichselbaum
patent: 3498860 (1970-03-01), Pickett
patent: 3546334 (1970-12-01), Lerner
patent: 3679450 (1972-07-01), Beightol
patent: 3891327 (1975-06-01), Welch
"Histological Techniques," Pease (Academic Press, 1964, pp. 82-89, 95-1100.
"Techniques for Electron Microscopy," Kay Blackwall Scientific, 1965, pp. 184-185.

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