Process for microencapsulating cells

Details Process for microencapsulating cells Process for microencapsulating cells

1997-07-10

2000-03-07

Lovering, Richard D.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

264 432, 264 433, 427 214, 424488, 424497, 424499, 435182, 514891, C12N 1110, A61K 950, B01J 1322

Patent

active

060338881

ABSTRACT:
Oxalate-degrading enzymes and bacteria were encapsulated for both enteric and intraperitoneal administration. We have shown that via alginate microencapsulation of Oxalobacter formigenes, enzymatic activity was retained for several months. A new process was developed which strengthened the aliginate microcapsules and their tolerance to citrate treatment. Much smaller (30-50 .mu.m) aliginate microcapsules were made for injection as means of impantation. For oral administration, multi-encapsulated microspheres of cellulose acetate phthalate in poly-2-vinylpyridine (pKa=3.5) were prepared to protect the enzymes from gastric juices.

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