Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1986-07-14
1989-03-14
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 18, 435 15, 435 25, 435805, 435810, C12Q 142, C12Q 100, C12Q 134, C12Q 148
Patent
active
048124001
ABSTRACT:
A process for measuring the sodium concentration of a biological fluid, such as blood serum or urine, is carried out by fixing the amounts of adenosine-5'-triphosphate (ATP), adenosine triphosphatase (ATPase), magnesium, and potassium, in the presence of a buffer, in enzymatic Reaction I, ##STR1## where ADP is adenosine diphosphate, and measuring the rate of appearance of the products so that the rate of reaction of adenosine triphosphatase can be determined. The reaction rate of this enzyme is directly proportional to the level of sodium present. The sodium concentration of the biological fluid being tested is quantitatively determined by comparing the reaction rate of adenosine triphosphatase to a standard known sodium concentration. Preferably, the reaction rate of adenosine triphosphatase is measured by reacting the ADP product with phosphoenolpyruvate under the catalysis of pyruvate kinase and measuring the pyruvate product of this second reaction. Quantitation of the pyruvate produced determines the activity of adenosine triphosphatase in Reaction I, which is directly proportional to the sodium concentration in the biological fluid being tested. Colorimetric standards of known sodium concentration are used as points of reference. A paper phase procedure for determining sodium levels in biological fluids is also provided.
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Kroll Michael I.
Scheiner Toni R.
Warden Robert J.
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