Process for measuring endotoxin

Details Process for measuring endotoxin Process for measuring endotoxin

1989-02-23

1993-01-12

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435 34, C12Q 137, C12Q 134, C12Q 104

Patent

active

051790068

ABSTRACT:
Measuring of endotoxin using a reaction of a horseshoe crab hemocyte lysate with endotoxin in a solution can be carried out in the presence of a water-soluble polysaccharide containing .beta.-1,3-glucosidic linkage and/or a water-soluble polysaccharide derivative containing .beta.-1,3-glucosidic linkage.

REFERENCES:
"Gelatin of Limulus Amoebocyte Lysate by an Antitumor (1.fwdarw.3)-.beta.-D-Glucan", Kakinuma et al, Biochemical and Biophysical Research Communications, vol. 101, No. 2, 1981, Jul. 30, 1981, pp. 434-439.
"Bacterial Endotoxin, Chemical, Biological and Clinical Aspects", Homma et al, Verlag Chemie, pp. 365-382.
"A New Chromogenic Endotoxin-Specific Assay Using Recombined Limulus Coagulation Enzymes and its Clinical Applications", Obayashi et al, Clinca Chemica Acta, 149 (1985), pp. 55-65.

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