Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1986-05-12
1988-08-02
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, C12Q 158
Patent
active
047613696
ABSTRACT:
A process for measuring the calcium concentration of a biological fluid is carried out by fixing the amounts of phospholipase A2 (PLA) and phosphatidylcholine (PC), in the presence of a buffer and a detergent such as sodium deoxycholate, in enzymatic Reaction I, ##STR1## and measuring the rate of appearance of the products so that the rate of reaction of PLA can be determined. The reaction rate of this enzyme is directly proportional to the level of calcium present. The calcium concentration of the biological fluid being tested is quantitatively determined by comparing the reaction rate of pohospholipase A2 to a standard of known calcium concentration. Preferably, the reaction rate of porcine pancreas phospholipase A2 is measured by determining the amount of lysolecithin produced in Reaction I by comparison of the optical density of the resultant color of coupled Reactions II and III,
REFERENCES:
patent: 4140579 (1979-02-01), Moncla
patent: 4168203 (1979-09-01), Takahashi
patent: 4657854 (1987-04-01), Wegfahrt
David Diagnostics, Inc.
Rosen Sam
LandOfFree
Process for measuring calcium levels in biological fluids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for measuring calcium levels in biological fluids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for measuring calcium levels in biological fluids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-711657