Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-10-19
1997-06-10
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 23, 435 34, C12Q 134, C12Q 104
Patent
active
056374747
ABSTRACT:
A process for measuring the amount of endotoxin or (1.fwdarw.3)-.beta.-D-glucan (referred to hereinafter as .beta.-glucan) contained in a sample, which comprises mixing the sample with amebocyte lysate of horseshoe crabs in the presence of at least one water-soluble polymer selected from the group consisting of a polyethylene glycol, a polyvinyl alcohol, a methyl cellulose and a hydroxypropyl cellulose, applying a light to the resulting mixture, measuring the time required until a degree of optical variation of the resulting mixture reaches a predetermined value after the mixing of the sample with the amebocyte lysate, or after the lapse of the predetermined time from the mixing of the sample with the amebocyte lysate, and determining the amount of endotoxin or .beta.-glucan contained in the sample on the basis of the relationship between the said time and the amount of endotoxin or .beta.-glucan; and also a reagent for measuring the amount of endotoxin or .beta.-glucan contained in a sample, which comprises amebocyte lysate of horseshoe crabs and at least one water-soluble polymer selected from the group consisting of a polyethylene glycol, a polyvinyl alcohol, a methyl cellulose and a hydroxypropyl cellulose.
REFERENCES:
patent: 4221865 (1980-09-01), Dubczak et al.
patent: 4221866 (1980-09-01), Cotter
patent: 4273557 (1981-06-01), Juranas
patent: 5318893 (1994-06-01), Matuura et al.
Database WPI, week 9539; Derwent Publications Ltd., London GB; AN 95-295935 & JP-A-07 191 038 (Wako Pure Chem Ind. Ltd.) 28 Jul. '95 (Abstract).
Ijiri Haruhisa
Kawabe Keiko
Takaoka Aya
Tsuchiya Masakazu
Gitomer Ralph
Wako Pure Chemical Industries Ltd.
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