Process for measuring activity of dehydrogenase employing a reac

Details Process for measuring activity of dehydrogenase employing a reac Process for measuring activity of dehydrogenase employing a reac

1984-12-27

1986-11-11

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435184, C12Q 132

Patent

active

046222960

ABSTRACT:
In a process for measuring the activity of a dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazolium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used as a reaction stopper, resulting in enhancement of coloring, stability of the produced color and prevention of the produced formazan compound from staining the laboratoryware.

REFERENCES:
patent: 3893890 (1975-07-01), Wurzburg et al.
patent: 3985621 (1976-10-01), Maruyama et al.

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