Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-26
1998-08-18
Houtteman, Scott W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
935 77, 935 78, C12P 1934
Patent
active
057957479
ABSTRACT:
The process of generating new biopolymers with improved characteristics, assisted by polymerases, is characterized in that at least one cycle of the steps described below, will be performed in a series of parallel preparations which are to be compared. Sequences of nucleic acids or, a mixture of similar sequences of nucleic acids in the distribution of mutants of a quasi-species, are subjected, in the region of the error threshold, to limited mutagenesis. These mixtures are replicated under simultaneous conditions and/or in succession. The mixtures of the so product nucleic acids are compartmentalized by segregation, and thereafter selected by a selection system which reflects the characteristics of interest of the nucleic acid sequence itself or, indirectly, via its translation product.
REFERENCES:
C. Turek et al., "Systematic evolution of Liands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase", Science, vol. 249, Aug. 3, 1990, pp. 505-510.
M. Eigen, "Macromolecular Evolution: Dynamical Ordering in Sequence Space", Berichte Der Bunsen-Gesellschaft Fur Physikalische Chemie, vol. 89, 1985, pp. 658-667.
D.L. Robertson et al., "Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA", Nature, vol. 344, Mar. 29, 1990, pp. 467-468.
G.F. Joyce, "Amplification, mutation and selection of catalytic RNA", Gene, vol. 82, 1989, pp. 83-87.
Eigen Manfred
Henco Karsten
Evotec BioSystems GmbH
Houtteman Scott W.
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