Process for manufacture of L-asparaginase from erwinia chrysanth

Details Process for manufacture of L-asparaginase from erwinia chrysanth Process for manufacture of L-asparaginase from erwinia chrysanth

1986-10-08

1988-03-08

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, 435847, C12N 982, C12R 118

Patent

active

047299576

ABSTRACT:
A process for the recovery and purification of L-asparaginase from Erwinia chrysanthemi is disclosed. The process involves the preparation of cellular acetone powder extract followed by either an ion exchange and affinity chromatography purification steps or by affinity chromatography alone. The column eluent is then dialyzed to produce substantially pure L-asparaginase.

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