Process for making yeast cells resistant to extreme high pressur

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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43525411, 43525421, C12N 1552, C12N 118, C12N 119, C12N 1509

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active

056747217

ABSTRACT:
Novel dividing cells of the yeast Saccharomyces. A first portion of dividing yeast cells is transformed with DNA encoding superoxide dismutase protein and DNA encoding catalase protein, and a second portion of yeast cells is not transformed with DNA grown at the same cell density as the first portion. When both portions of cells are heated in the presence of oxygen containing gas to a temperature of 50 degrees Celsius and are maintained at such temperature for 20 minutes, at least twice as many cells of the first portion of cells survive.

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Bermingham McDonogh et al (1988) "The copper, zinc superoxide dismitase gene of saccharomyces derivisea: Cloning, sequencing and biological activity": Proc. Natl. Acad. Sci 85:4789-4793.
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Kono et al. (1982) "Superoxide radical inhibits catalase", J. Biol. Chem. 257:5751-5754.
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