Process for making T cell hybridomas

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 702, 4352402, 43524021, 43524025, 43524026, 43524031, 530351, 530412, 530809, 935101, 935 93, 935 95, C12N 1500, C12N 500, C12N 502

Patent

active

049505980

ABSTRACT:
Human T-T hybridomas are made by fusing an azaserine-hypoxanthine (AH) sensitive T leukemia cell line, preferably the AH-sensitive mutant of the Jurkat leukemia line identified as J3R7, with normal T cells and culturing the fusion product in a selective AH medium. Stable, interleukin-2 (IL-2)-producing human T-T hybridomas were made by this process.

REFERENCES:
patent: 4350683 (1982-09-01), Galfre et al.
patent: 4364937 (1982-12-01), Kung et al.
patent: 4411993 (1983-10-01), Gillis
patent: 4473642 (1984-09-01), Gillis
patent: 4490289 (1984-12-01), Stern
Gillis et al., J. Exp. Med., 152, 1709-19, (1980).
Harwell et al., J. Exp. Med., 152, 893 (1980).
Kamatani et al., PNAS (U.S.A.), 78(2), 1219-23, (Feb. 1981).
Lakow, E. et al., J. Cell. Biochem. Suppl., No. 6, p. 77 (1982). Oral Disclosure 2/28-3/5, 1982, Bio. Abs. 27010783.
Lakow, E. et al., Fed. Proc., vol. 41 (3), abs. 1919 (1982), Oral Disclosure of Apr. 15-23, 1982, Bio. Abs. 23044014J.
Cox, R. et al., Mutation Res., vol. 36(1), pp. 93-103 (1976), Bio. Abs. 62060609.
Pawalec, G. et al., Eur. J. Immunol., vol. 12(5), pp. 387-392. Bio. Abs. 75010764.
Gillis, S. et al., J. Exp. Med., vol. 152 (6), pp. 1709-1719 (1980). Bios. Abst. 71059830.
Frank, M. B. et al., J. of Immunology, vol. 127 (6), pp. 2361-2365 (1981). Bio. Abst. 74011171.
Irigoyen, O. et al., J. Exp. Med., vol. 154, pp. 1827-1837, (1981), "Generation of Functional Human T Cell Hybrids".
Chemical Abstracts, vol. 98, abstract no. 105727 w, 1983, Yamamura, Y. et al., "Human T-Cell Lines." (12 Jun. 1981).
Chemical Abstracts, vol. 98, abstract no. 874043, 1983, Kishimoto, T. et al., "Immunoregulatory Molecules (Lymphokines) Secreted from Human Monoclonal T Cell Lines".
Lakow, E. et al., Journal of Immunology, vol. 130, No. 1, pp. 169-172, (1983), "Human T Cell Hybridomas Specific for Epstein Barr Virus Infected B Lymphocytes".
DeFreitas, E. C. et al., Proc. Natl. Acad. Sci. U.S.A., vol. 79, pp. 6646-6650 (1982), "Antigen-Specific Human T-Cell Hybridomas with Helper Activity".
Greene, W. C. et al., Clinical Research, vol. 29, p. 368A (1981), "Production and Characteristics of Human T Cell Hybrids Exhibiting Suppressor Cell Activity".
Croce, C. M. et al., Nature, vol. 288, pp. 488-409 (1980), "Production of Human Hybridomas Secreting Antibodies to Measles Virus".
Grillot-Courvalin, C. et al., Nature, vol. 292, pp. 844-845 (1981), "Establishment of a Human T-Cell Hybrid Line with Suppressive Activity".
Okada, M. et al., Proc. Natl. Acad. Sci. U.S.A., vol. 78, No. 12, pp. 7717-7721 (1981), "Establishment and Characterization of Human T Hybrid Cells Secreting Immunoregulatory Molecules".
Kohler, G. et al., Nature, vol. 256, pp. 495-497 (1975), "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity".
Olsson, L. et al., Proc. Natl. Acad. Sciences U.S.A., vol. 77, No. 9, pp. 5429-5431 (1980), "Human-Human Hybridomas Producing Monoclonal Antibodies of Predefined Antigenic Specificity".
Levy, R. et al., Proc. Natl. Acad. Sci. U.S.A., vol. 75, No. 5, pp. 2411-2415 (1978), "Rescue of Immunoglobulin Secretion from Human Neoplastic Lymphoid Cells by Somatic Cell Hybridization".
Kappler, J. W. et al., J. Exp. Med., vol. 153, pp. 1198-1214 (1981), "Antigen-Inducible, H-2 Restricted, Interleukin-2-Producing T Cell Hybridomas".
Fox, R. M. et al., Cancer Research, vol. 40, pp. 1718-1721 (1980), '

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Process for making T cell hybridomas does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for making T cell hybridomas, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for making T cell hybridomas will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1677242

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.