Process for making dust-free enzyme-containing particles from an

Details Process for making dust-free enzyme-containing particles from an Process for making dust-free enzyme-containing particles from an

1995-02-15

1998-09-29

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

435176, 435178, 435187, 510530, C12N 1100, C12N 998, C12N 1110, C11D 742

Patent

active

058145010

ABSTRACT:
Substantially dust-free enzyme-containing particles are prepared by spray-coating solid particulate cores with a fermentation broth containing enzymes. Fermentation broths containing from 5% to 100% total enzyme solids and 4%-55% total solids content in the broth may be used. For broths containing 4%-31% total solids, enzyme and coating agents may be spray-coated so as to achieve a weight gain of 1%-24% w/w over the initial weight of the cores. For broths with 31%-55% total solids, a corresponding weight gain of 1%-100% may be achieved. The enzyme coated cores may be further coated with coating agents. The total dry weight added to the cores ranges from 1% to 19% in the absence of coating agents and 1% to 24% in the presence of coating agents when the lower range of total solids content is used. The total dry weight added to the cores ranges from 1% to 100% when the higher range of total solids content is used. These conditions are especially useful in the granulation of enzymes with very high specific activities, so that effective enzyme activity can be provided by adding low amounts of broth solids to the cores. Reduction in added solids is of significant economic benefit in reducing granulation costs.

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