Process for making and isolating cyclosporin a by fermentation

Details Process for making and isolating cyclosporin a by fermentation Process for making and isolating cyclosporin a by fermentation

1991-04-22

1993-10-26

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 713, 435803, 435911, 435171, 4352541, 530317, 530321, C12P 2100, C12P 2104, C12R 1645

Patent

active

052565475

ABSTRACT:
Method of fermentative production and isolation of Cyclosporin A by aerobic fermentation of a fungus microorganism in a medium containing mineral salts, a nitrogen-containing substrate, including using a producer strain consisting of strain Wb 6-5 of Tolypocladium inflatum, IMET 43 899 Sesquicilliopsis rosariensis G.ARNOLD, IMET 43 888, Sesquicilliopsis rosariensis G.ARNOLD F605, IMET 43 887, a mutant thereof or a strain obtained therefrom by selection; culturing the producer strain in a culture medium containing an ammonium salt as a single source of nitrogen; mixing the culture medium with a filter aid to form a suspension; after the mixing step, filtering the suspension to form a filter cake containing moist biomass and filter aid; drying the filter cake after the filtering; after the drying step, extracting the filter cake with a supercritical gas, advantageously carbon dioxide, or a lower carboxylic acid ester to form an extract; distributing the extract between aqueous methanol solution and petroleum ether to remove fats and form a purified extract; and chromatographing the purified extract by preparative HPLC using silica gel or aluminum oxide.

REFERENCES:
patent: 4117118 (1978-09-01), Harri et al.
patent: 4215199 (1980-07-01), Harri et al.
patent: 4288431 (1981-09-01), Traber et al.
patent: 4289851 (1981-09-01), Traber et al.

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