Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-06
1998-06-16
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, C12Q 168, C07H 2104
Patent
active
057668523
ABSTRACT:
A process for lysing Mycobacteria to liberate nucleic acids such as DNA and RNA comprises heating the Mycobacteria in an aqueous solution to lyse the Mycobacteria and release the nucleic acids, with the aqueous solution containing a chelating agent such as EDTA or EGTA in an amount effective to inhibit degradation of the released nucleic acids. Examples of Mycobacteria which can be lysed include Mycobacterium fortuitum and Mycobacterium tuberculosis. After lysis, the nucleic acid is preferably then amplified and detected. Kits useful for carrying out the present invention are also disclosed.
REFERENCES:
J. V. Iralu, et al. "Diagnosis of Mycobacterium avium bacteremia by polymerase chain reaction" J. Clin. Microbiol. 31:1811-1814 (1993).
Y. Mizuguchi and T. Tokunaga "Method for isolating deoxyribonucleic acid from mycobacteria" J. Bacteriol. 104:1020-1021 (1970).
K. Dix, et al. "Species-specific oligonucleotide probes for the identification of periodontal bacteria" J. Clin. Microbiol. 28:319-323 (1990).
Sigma Catalog (1992) p. 412.
Maniatis et al "Molecular Cloning" 1982.
D. Thierry, et al., The Detection of Mycobacterium Tuberculosis in Uncultured Clinical Specimens Using the Polymerase Chain Reaction and a Non-radioactive DNA Probe, Mol. Cellular Probes, (1992) 6:181-191.
V.Sritharan, et al., A Simple Method for Diagnosing M. Tuberculosis Infection in Clinical Samples Using PCR, Mol. Cellular Probes, (1991) 5:385-395.
A.H. J. Kolk, et al., Detection of Mycobacterium Tuberculosis in Clinical Samples by Using Polymerase Chain Reaction and a Nonradioactive Detection System, Journal of Clinical Microbiology, (Oct. 1992) 30:2567-2575.
T. Victor, et al., Purification of Sputum Samples Through Sucrose Improves Detection of Mycobacterium Tuberculosis by Polymerase Chain Reaction, Journal of Clinical Microbiology, (Jun. 1992) 30:1514-1517.
K. Eisenach, et al., Detection of Mycobacterium Tuberculosis in Sputum Samples Using a Polymerase Chain Reaction, Am. Rev Respir. Dis., 144:1160-1163.
D. Cousins, et al., Use of Polymerase Chain Reaction for Rapid Diagnosis of Tuberculosis, Journal of Clinical Microbiology, 30:255-258.
P. Del Portillo, et al., Amplification of Species-Specific DNA Fragment of Mycobacterium Tuberculosis and its Possible Use in Diagnosis, Journal of Clinical Microbiology, (Oct. 1991) 29:2163-2168.
G. Buck, et al., Rapid, Simple Method for Treating Clinical Specimens Containing Mycobacterium Tuberculosis to Remove DNA for Polymerase Chain Reaction, Journal of Clinical Microbiology, (May 1992) 30:1331-1334.
B. Savic, et al., Evaluation of Polymerase Chain Reaction, Tuberculostearic Acid Analysis, and Direct Microscopy for the Detection of Mycobacterium Tuberculosis in Sputum, Concise Communications, JID 1992:166 (Nov.) 1177-1180.
P. Shankar, et al., Rapid Diagnosis of Tuberculosis Meningitis by Polymerase Chain Reaction, The Lancet, (Jan. 5, 1991) 337:5-7.
A. Brisson-Noel, et al., Rapid Diagnosis of Tuberculosis by Amplification of Mycobacterial DNA in Clinical Samples, The Lancet, (Nov. 4, 1989) 1069-1071.
D. De Wit, et al., Direct Detection of Mycobacterium Tuberculosis in Clinical Specimens by DNA Amplification, Journal of Clinical Microbiology, (Nov. 1990) 28:2437-2441.
U. Sjobring, et al., Polymerase Chain Reaction for Detection of Mycobacterium Tuberculosis, Journal of Clinical Microbiology, (Oct. 1990) 28:2200-2204.
B.B. Plikaytis, et al., Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay, Molecular and Cellular Probes 5, 215-219.
Down James A.
Fraiser Melinda S.
Walker G. Terrance
Becton Dickinson and Company
Campbell Eggerton A.
Fugit Donna R.
LandOfFree
Process for lysing mycobacteria does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for lysing mycobacteria, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for lysing mycobacteria will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1724021