Process for lysing mycobacteria

Details Process for lysing mycobacteria Process for lysing mycobacteria

1997-07-28

1999-01-05

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12P 1934, C12Q 168

Patent

active

058561455

ABSTRACT:
A process for lysing Mycobacteria to liberate nucleic acids such as DNA and RNA comprises heating the Mycobacteria in an aqueous solution to lyse the Mycobacteria and release the nucleic acids, with the aqueous solution containing a chelating agent such as EDTA or EGTA in an amount effective to inhibit degradation of the released nucleic acids. Examples of Mycobacteria which can be lysed include Mycobacterium fortuitum and Mycobacterium tuberculosis. After lysis, the nucleic acid is preferably then amplified and detected. Kits useful for carrying out the present invention are also disclosed.

REFERENCES:
patent: 5702884 (1997-12-01), Ekeze et al.
patent: 5712095 (1998-01-01), Britschgi et al.
Savic et al. J.I.D 166:1177-1180, 1992.
Sritharan et al. Mol. Cell. Probes 5: 385-395, 1991.

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