Process for lysing Mycobacteria

Details Process for lysing Mycobacteria Process for lysing Mycobacteria

1994-08-09

1996-04-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435259, C12Q 168, C12P 1934, C12N 106

Patent

active

055124402

ABSTRACT:
The invention provides a rapid process for lysing Mycobacteria. In one embodiment is provided a process for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of heat. A particularly effective method for providing the necessary heat is in the form of forced hot air such as in a forced hot air oven. The process of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.

REFERENCES:
patent: 5376527 (1994-12-01), Robson et al.
Kolk et al, "Detection of Mycobacterium tuberculosis in Clinical Samples by Using Polymerase Chain Reaction and a Nonradioactive Detection System," J. of Clinical Microbiology, 30: 2567-2575, 1992.

Affiliated with

Also associated with

Rating

Process for lysing Mycobacteria does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for lysing Mycobacteria, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for lysing Mycobacteria will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-627755