Process for liberating an analyte from its binding protein

Details Process for liberating an analyte from its binding protein Process for liberating an analyte from its binding protein

1990-01-10

1994-07-26

Housel, James C.

Chemistry: analytical and immunological testing

Including sample preparation

Digestion or removing interfering materials

436174, 436 86, 436119, G01N 100

Patent

active

053326784

ABSTRACT:
A process for the dissolving off of an analyte bound to a binding protein by breakdown of the binding protein using a cleaving agent which cleaves --S--S-- groups into SH groups in the alkaline range, wherein a 1,2-dithiolan-3-carboxylic acid of the formula: ##STR1## is used as the --S--S-- group cleaving agent in which n is a whole number of from 1 to 8. The process is used for preparing samples for analytical or investigational processes for the determination of a liberated analyte and especially for immunoassays for vitamins such as vitamin B.sub.12, steroids such as testosterone or for thyroid hormones such as T.sub.3 /T.sub.4.

REFERENCES:
patent: 4100150 (1978-07-01), Cartwright
patent: 4429008 (1984-01-01), Martin et al.
patent: 4545988 (1985-10-01), Nakayama et al.
patent: 4714611 (1987-12-01), Yasaburgo et al.
patent: 4767204 (1988-08-01), Cleveland
patent: 4780281 (1988-10-01), Marnett et al.
patent: 4784804 (1988-11-01), Basch et al.
patent: 4806524 (1989-02-01), Kawaguchi et al.
patent: 4812557 (1989-03-01), Yasushi et al.
patent: 4902495 (1990-02-01), Kaliner et al.
patent: 4978613 (1990-12-01), Bieniarz et al.
patent: 4981979 (1991-01-01), Sivam
The Condensed Chemical Dictionary-10th Edition, 1981 p. 1019.
The Merck Index-10th Edition, 1983, pp. 493 and 1336.
Silverman, R. B.; Nandi, D. L.; "Reduced Thioredoxin: A Possible Physiological Cofactor for Vitamin K Epoxide Reductase. Further Support for an Active Site Disulfide", Biochem. Biophys. Res. Commun, 155(3) pp. 1248-1254 Sep. 30, 1988.
Pettit, F. H.; Humphreys 5; Reed, L.J.; "Regulation of Pyruvate Dehydrogenase Kinase Activity by Protein Thiol-Disulfide Exchange"; Proc Natl Acad Sci USA, (1982 Jul) 79(13) 3945-8.
STN-File Biosis Accession No. 76:226983 Iordan et al; "Ribo Nucleotide Reductase of Propioni-Bacterium-Shermanii"; Mikrobiologiya 44(4)-1975 pp. 609-614.

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