Process for labeling single-stranded nucleic acids and hybridiza

Details Process for labeling single-stranded nucleic acids and hybridiza Process for labeling single-stranded nucleic acids and hybridiza

1986-01-09

1989-04-18

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 436501, 436827, 536 27, 935 78, C12Q 168

Patent

active

048227315

ABSTRACT:
Nucleic acids may be labeled by complexing the alkylating moiety of a labeling reagent into a single-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a single-stranded hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:

REFERENCES:
patent: 4124598 (1978-11-01), Hearst et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4542102 (1985-09-01), Dattagupta et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4582788 (1986-04-01), Erlich
patent: 4582789 (1986-04-01), Sheldon et al.
patent: 4617261 (1986-10-01), Sheldon et al.
patent: 4683195 (1987-07-01), Mullis et al.
Sood et al., PNAS, 78, 616-620 (1981).
Ploegh et al., PNAS, 77, 6081-6085 (1980).
Brown et al., GENE, 20, 139-144 (1982).
Manning et al., CHROMOSOMA, 53, 107 (1975).
Langer et al., Proc. Natl. Acad. Sci. USA, 78, 6633-6637 (1981).
Leary et al., Proc Natl. Acad. Sci. USA 80, 4045-4049 (1983).
Song et al., ANYAS (1980) 355-67.
Hyde et al., Biochemistry, 17 (1978) 1251-7.
Saffran et al., Proc. Natl. Acad. Sci. U.S.A. 79, 4594-4598 (1982).
V. G. Dev et al., Lancet (England) 1, 1285 (Jun. 10, 1972).
Schwartz et al., Cold Spring Harbor Laboratory Symposium XLVII, pp. 189-195.

Affiliated with

Also associated with

Rating

Process for labeling single-stranded nucleic acids and hybridiza does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for labeling single-stranded nucleic acids and hybridiza, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for labeling single-stranded nucleic acids and hybridiza will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2396700