Process for labeling acridinium to microparticles and applicatio

Details Process for labeling acridinium to microparticles and applicatio Process for labeling acridinium to microparticles and applicatio

1994-02-15

1995-11-21

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436528, 436531, 436534, 422 52, 427 21, 427 211, 427 213, G01N 33543, G01N 33546

Patent

active

054686490

ABSTRACT:
Embodiments of the invention provide methods of preparing an activated acridinium microparticle. Generally, the methods involve direct covalent coupling or an affinity format. The direct covalent coupling method involves coating a microparticle with a proteinaceous compound. Then, a 10-methyl-N-tosyl-N-(2-carboxyethyl)-9-acridinium carboximide trifluoromethane sulfonate is coupled to the proteinaceous compound. In the affinity format, a microparticle is coated with a biotinylated proteinaceous compound. The microparticle is reacted with an anti-biotin labelled 10-methyl-N-tosyl-N-(2-carboxyethyl)-9-acridinium carboximide trifluoromethane sulfonate. Methods are also provided for using such a microparticle. Those methods of use can estimate transfer efficiency, calibrate optics, and measure membrane pore size of a chemiluminescence based instrument. Test elements for analytical instruments are also provided.

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