Process for labeling a nucleic acid

Details Process for labeling a nucleic acid Process for labeling a nucleic acid

2005-06-07

2005-06-07

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S025300, C536S025310, C540S465000, C540S474000, C556S001000, C556S006000

Reexamination Certificate

active

06902891

ABSTRACT:
A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4868105 (1989-09-01), Urdea et al.
patent: 5124246 (1992-06-01), Urdea et al.
patent: 5130238 (1992-07-01), Malek et al.
patent: 5171853 (1992-12-01), Thorp et al.
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5317098 (1994-05-01), Shizuya et al.
patent: 5328824 (1994-07-01), Ward et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5407797 (1995-04-01), Marliere et al.
patent: 5422252 (1995-06-01), Walker et al.
patent: 5437990 (1995-08-01), Burg et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5449767 (1995-09-01), Ward et al.
patent: 5512430 (1996-04-01), Gong
patent: 5525464 (1996-06-01), Drmanac et al.
patent: 5554516 (1996-09-01), Kacian et al.
patent: 5582829 (1996-12-01), Alliger et al.
patent: 5585481 (1996-12-01), Arnold, Jr. et al.
patent: 5605796 (1997-02-01), Chen et al.
patent: 5684149 (1997-11-01), Morrow
patent: 5688670 (1997-11-01), Szostak et al.
patent: 5700637 (1997-12-01), Southern
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5766849 (1998-06-01), McDonough et al.
patent: 5807522 (1998-09-01), Brown et al.
patent: 5981734 (1999-11-01), Mirzabekov
patent: 5989904 (1999-11-01), Das et al.
patent: 6297010 (2001-10-01), Stefano
patent: 6376179 (2002-04-01), Laayoun
patent: A 3 910 151 (1990-10-01), None
patent: 198 15 864 (1999-10-01), None
patent: A 0 063 879 (1982-11-01), None
patent: A 0 097 373 (1984-01-01), None
patent: A 0 280 058 (1988-08-01), None
patent: A 0 286 898 (1988-10-01), None
patent: A 0 302 175 (1989-02-01), None
patent: A 0 320 308 (1989-06-01), None
patent: A 0 329 198 (1989-08-01), None
patent: A 0 567 841 (1993-11-01), None
patent: A 0 709 468 (1996-05-01), None
patent: 0 801 072 (1997-10-01), None
patent: 2 768 743 (1999-03-01), None
patent: 2 781 500 (2000-01-01), None
patent: WO A 88/01302 (1988-02-01), None
patent: WO A 88/04300 (1988-06-01), None
patent: WO A 88/10315 (1988-12-01), None
patent: WO A 90/14439 (1990-11-01), None
patent: WO A 93/16094 (1993-08-01), None
patent: WO 93/20241 (1993-10-01), None
patent: WO A 93/22461 (1993-11-01), None
patent: WO A 94/03472 (1994-02-01), None
patent: WO A 94/29723 (1994-12-01), None
patent: WO A 95/03142 (1995-02-01), None
patent: WO A 95/03430 (1995-02-01), None
patent: WO A 95/08000 (1995-03-01), None
patent: WO A 95/11995 (1995-05-01), None
patent: WO A 96/19729 (1996-06-01), None
patent: WO A 96/28460 (1996-09-01), None
patent: WO A 98/05766 (1998-02-01), None
patent: WO A 98/11104 (1998-03-01), None
patent: WO 98/27229 (1998-06-01), None
patent: WO 99/65926 (1999-12-01), None
Chee, M. et al., “Accessing Genetic Information with High-Density DNA Arrays,” Science, vol. 274, pp. 610-614, 1996.
Caviani Pease, A. et al., “Light-generated oligonucleotide arrays for rapid DNA sequence analysis,” Proc. Natl. Acad. Sci. USA, vol. 91, pp. 5022-5026, 1994.
Sambrook, J. et al., “Molecular Cloning, a Laboratory Manual,” Cold Spring Harbor Laboratory Press, Second Edition, pp. 5.30-5.95, 1989.
Lindahl, T. et al., “Rate of Chain Breakage at Apurinic Sites in Double-Stranded Deoxyribonucleic Acid,” Biochemistry, vol. 11, No. 19, pp. 3618-3623, 1972.
Liuzzi, M. et al., “Characterization of damage in γ-irradiated and OsO4-treated DNA using methoxyamine,” Int. J. Radiat. Biol., vol. 54, No. 5, pp. 709-722, 1988.
Breslow, R. et al., “Recognition and catalysis in nucleic acid chemistry,” Proc. Natl. Acad. Sci. USA, vol. 90, pp. 1201-1207, 1993.
Hovinen, J. et al., “Imidazole Tethered Oligodeoxyribonucleotides: Synthesis and RNA Cleaving Activity,” J. Org. Chem., vol. 60, pp. 2205-2209, 1995.
Blattner, F. et al., “The Complete Genome Sequence ofEscherichia coliK-12,” Science, vol. 277, pp. 1453-1462, 1997.
Abravaya, K. et al., “Strategies to Avoid Amplicon Contamination,” Nucleic Acid Amplification Technologies, pp. 125-133, 1997.
Chevalier, J. et al., “Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes: Where Do We Stand?” J. Histochem. Cytochem., vol. 45, No. 4, pp. 481-491, 1997.
Greisen, K. et al., “PCR Primers and Probes for the 16S rRNA Gene of Most Species of Pathogenic Bacteria, Including Bacteria Found in Cerebrospinal Fluid,” J. Clinical Microbiol., vol. 32, No. 2, pp. 335-351, 1994.
Troesch, A. et al., “MycobacteriumSpecies Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays,” J. Clinical Microbiol., vol. 37, No. 1, pp. 49-55, 1999.
Furuta, T. et al., “Direct Esterification of Phosphates with Various Halides and its Application to Synthesis of cAMP Alkyl Triesters,” J. Chem. Soc. Perkin Trans., vol. 1, pp. 3139-3142, 1993.
Sambrook, J. et al., “Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients,” Plasmid Vectors, 2ndEdition, p. 1.46, 1989.
Zuckermann, R. et al., “Site-selective cleavage of structured RNA by a staphylococcal nuclease-DNA hybrid,” Proc. Natl. Acad. Sci. USA, vol. 86, pp. 1766-1770, 1989.
Gish, G. et al., “DNA and RNA Sequence Determination Based on Phosphorothioate Chemistry,” Science, vol. 240, pp. 1520-1522, 1988.
Nakamaye, K.L. et al., “Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside α-thiotriphosphates,” Nucleic Acids Res., vol. 16, No. 21, pp. 9947-9959, 1988.
Ruffner, D.E. et al., “Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction,” Nucleic Acids Res., vol. 18, No. 20, pp. 6025-6029, 1990.
Almer, H. et al., “Nonenzymatic Hydrolysis of an RNA-dimer Containing a Thiophosphate Linkage,” Nucleosides & Nucleotides, vol. 10, pp. 653-655, 1991.
Mag, M. et al., “Synthesis and selective cleavage of an oligodeoxynucleotide containing a bridged internucleotide 5′-phosphorothioate linkage,” Nucleic Acids Res., vol. 19, No. 7, pp. 1437-1441, 1991.
Vyle, J.S. et al, “Sequence- and Strand-Specific Cleavage in Oligodeoxyribonucleotides and DNA Containing 3′-Thiothymidine,” Biochemistry, vol. 31, pp. 3012-3018, 1992.
Podyminogin, M.A. et al., “Synthetic RNA-cleaving molecules mimicking ribonuclease A active center. Design and cleavage of tRNA transcripts,” Nucleic Acids Res., vol. 21, No. 25, pp. 5950-5956, 1993.
Kuimelis, R.G. et al., “Cleavage properties of an oligonucleotide containing a bridged internucleotide 5′-phosphorothioate RNA linkage,” Nucleic Acids Res., vol. 23, No. 23, pp. 4753-4760, 1995.
Fidanza, J.A. et al., “Site-Specific Labeling of DNA Sequences Containing Phosphorothioate Diesters,” J. Am. Chem. Soc., vol. 114, pp. 5509-5517, 1992.
Vlassov, V.V. et al., “Cleavage of tRNA with imidazole and spermine imidazole constructs: a new approach for probing RNA structure,” Nucleic Acids Res., vol. 23, No. 16, pp. 3161-3167, 1995.
Ramsay, G., “DNA chips: State-of-the-art,” Nature Biotechnol., vol. 16, pp. 40-44, 1998.
Ginot, F., “Oligonucleotide Micro-Arrays for Identification of Unknown Mutations: How Far from Reality?” Human Mutation, vol. 10, pp. 1-10, 1997.
Cheng, J. et al., “Microc

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