Process for isolation of the B oligomer of pertussis toxin

Details Process for isolation of the B oligomer of pertussis toxin Process for isolation of the B oligomer of pertussis toxin

1987-02-03

1989-07-04

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

424 92, 435183, 435193, 530403, 530415, 530416, 530825, C12N 912

Patent

active

048450367

ABSTRACT:
A method for dissociating the B oligomer of pertussis toxin comprising incubating pertussis toxin in an aqueous solution of urea, sodium phosphate buffer, and a nucleotide selected from the group consisting of ATP and ADP, and optional zwitterionic detergent; applying the incubated solution to a CM-Sepharose column; and eluting the B oligomer from the column with potassium phosphate buffer containing urea.

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Wong et al., "Pertussis Toxin Substrate is a Guanosine 5'-[.beta.-thio] Diphosphate-N-Ethylmaleimide--, Mg.sup.+2 -and Temperature-Sensitive GTP-Binding Protein," Biochem. J., 1985, 232(1), 191-7.
Burns et al., "Adenine Nucleotides Promote Dissociation of Pertussis Toxin Subunits", J. Biol. Chem., 1986, 261(9), 4324-7.
Biochemistry (1982), vol. 21, pp. 5516-5522.

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