Process for isolation of ribulose 1,5-diphosphate carboxylase fr

Details Process for isolation of ribulose 1,5-diphosphate carboxylase fr Process for isolation of ribulose 1,5-diphosphate carboxylase fr

1979-09-24

1981-05-19

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435814, 435816, C12N 988

Patent

active

042686326

ABSTRACT:
Described herein is a process for isolating the enzymatic protein ribulose 1,5-diphosphate carboxylase from the green leaves of plants. In the process, which is particularly suited to obtaining the protein from tobacco, the leaves are ground or otherwise pulverized in the presence of a reducing agent, followed by heating the resulting pulp to about 50.degree. C. A liquid portion containing the desired protein is separated from the pulp and then cooled to cause the crystallization of the ribulose 1,5-diphosphate carboxylase. After separation of the crystalline material, the supernatant is acidified to yield lower molecular weight proteins.

REFERENCES:
Barman, Enzyme Handbook, vol. II, pp. 723-724 (1969).
Kawishima et al., Biochimica Biophysica Acta, vol. 229, pp. 749-760 (1971).

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