Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1981-05-15
1982-08-31
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
435816, C12N 988
Patent
active
043473245
ABSTRACT:
Described herein is a process for isolating the enzymatic protein ribulose 1,5-diphosphate carboxylase from the green leaves of plants. In the process, which is particularly suited to obtaining the protein from tobacco, the leaves are ground or otherwise pulverized in the presence of a reducing agent. A liquid portion containing the desired protein is separated from the pulp and its pH adjusted to within the range of from about pH 6.0 to 5.3 and then cooled to cause the crystallization of the ribulose 1,5-diphosphate carboxylase. After separation of the crystalline material, the supernatant is acidified to yield lower molecular weight proteins.
REFERENCES:
patent: 2600903 (1932-06-01), Miller
patent: 3780183 (1973-12-01), Edwards et al.
patent: 3823128 (1974-07-01), Bickoff et al.
Kung et al., in Methods in Enzymology, vol. 69, pp. 326-336, (1980).
Chan et al., Science, vol. 176, pp. 1145-1146, (1972).
Johal et al., Science, vol. 204, pp. 75-77, (Apr. 1979).
Kwanyuen Prachuab
Wildman Samuel G.
Leaf Proteins, Inc.
Shapiro Lionel M.
LandOfFree
Process for isolation of proteins from plant leaves does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for isolation of proteins from plant leaves, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for isolation of proteins from plant leaves will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2184797