Process for isolation of osteopontin from milk

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

Reexamination Certificate

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C530S350000, C530S412000, C530S415000, C530S418000

Reexamination Certificate

active

10168303

ABSTRACT:
A process for isolation of milk osteopontin from a material containing milk osteopontin by optionally mixing the milk material with a calcium source and separate the osteopontin containing phase from the rest of the milk material by pH adjustment.

REFERENCES:
patent: 0 404 425 (1990-12-01), None
Kunz C and Lonnerdal B Human milk proteins: separation of whey proteins and their analysis by polyacrilymide gel electrophoresis, fast protein liquid chromatography (FPLC) gel filtration, and anion exchange chromatography. An J Clin Nut. 1989 (49):464-70.
Bayless KJ, Davis GE, Meininger GA Isolation and Biological Properties of Osteopontin from Bovine Milk. Protein Expression and Purification. 1997.(9):309-314.
Prince CW, Oosawa T, Butler WT, Tomana M, Bhown AS, Bhown M Schrohenloher RE Isolation, Characterization and Biosynthesis of a Phosphorylated Glycoprotein from Rat Bone. J Biol Chem (1987) 262:2900-2907.
Sorensen ES, Petersen TE Purification and charaterization of three proteins isolated from the protease fraction of bovine milk. J Dairy Res (1993) 60:189-97. Abstract.
Sorensen S., Justensen, S.J., Johnsen, A.H. Identification of a macromolecular crystal growth inhibitor in human urine as osteopontin. Urol Res (1995) 23(5):327-34 (abstract).
Zhi Yong Ju et al., “Gelation of pH-aggregated Whey Protein Isolate Solution Induced by Heat, Protease, Calcium Salt, and Acidulant”,J. Agric. Food Chem., vol. 46, pp. 1830-1835, (1998).
Erdjan Salih et al., “Identification of the Phosphorylated Sites of Metabolically32P-Labeled Osteopontin From Cultured Chicken Osteoblasts”,The Journal of Biological Chemistry, vol. 272, No. 21, pp. 13966-13973, (May 1997).
Kayla Bayless et al., “Isolation and Biological Properties of Osteopontin From Bovine Milk”, Abstract from Protein Expression and Purification, vol. 9, Database accession No. PREV199799513298, XP002146905, (Jul. 1997).

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