Process for isolation of insulin from plant source

Details Process for isolation of insulin from plant source Process for isolation of insulin from plant source

1974-07-16

1976-03-23

Roberts, Elbert L.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

195 4, A61K 3726, C12B 120

Patent

active

039459888

ABSTRACT:
A method for isolating insulin from the cultured seeds or fruit of Memordica charantia Linn in which the starting material is extracted with ethanol, water and sulfuric acid, adjusting the pH of the extract to 1.5 to 2.0 and then precipitating the insulin by adding cold ethanol and diethyl ether. Zinc traces are added to precipitate the insulin as white crude crystals which are then purified to white needle-like crystals. Comparison of this product with standard insulin by chromatography showed that the present product is substantially the same as the standard insulin.

REFERENCES:
prout: Metab., 12, 673-686 (1963).
Steiner et al.: Proc. Nat. Acad. Sci. (USA), 57, 473-480 (1967).

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