Process for isolation of carnitinehydrolyase and an effector fro

Details Process for isolation of carnitinehydrolyase and an effector fro Process for isolation of carnitinehydrolyase and an effector fro

1988-10-25

1990-03-06

Rosen, Sam

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435136, 435280, C12P 1300

Patent

active

049065688

ABSTRACT:
An enzyme carnitinehydrolyase is isolated from microorganisms belonging to the family Enterobacteriaceae, particularly Escherichia coli, Proteus vulgarius, Proteus mirabilis, Citrobacter freundii, Salmonella typhimurium, Salmonella anatum, e Salmonella cottbus, following anaerobic cultivation of the microorganisms in the presence of D,L-carnitine and/or crotonobetaine, by cell disruption and chromatography of the resulting protein extract. The enzyme, upon immobilization and activation by an effector which is also obtained from the protein extract following enzyme isolation, is used for stereoselectively hydrating crotonobetaine into L(-)-carnitine by contacting a solution of crotonobetaine or crotonobetaine derivatives with carnitinehydrolyase in the presence of the effector.

REFERENCES:
patent: 4650759 (1987-03-01), Yokozeki et al.
Fukui et al--Chem. Abst. vol. 105 (1986) p. 132142U.

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