Process for isolating vascular endothelial cells from...

Details Process for isolating vascular endothelial cells from... Process for isolating vascular endothelial cells from...

2011-04-05

2011-04-05

Ketter, James S (Department: 1636)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

C435S366000

Reexamination Certificate

active

07919318

ABSTRACT:
The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.

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