Process for isolating superoxide dismutase from red blood cells

Details Process for isolating superoxide dismutase from red blood cells Process for isolating superoxide dismutase from red blood cells

1981-06-19

1982-08-24

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435192, 435816, 260113, C12N 902

Patent

active

043461743

ABSTRACT:
A process for isolating superoxide dismutase from red blood cells comprising the steps of heating hemolyzed red blood cells in the presence of at least one monovalent inorganic neutral salt and at least one transition metal salt; eliminating precipitate from the hemolyzed red blood cells by filtration or centrifugation to obtain a solution; adding further at least one transition metal salt at least once to the solution under application of heat thereto; and eliminating precipitate from the solution.

REFERENCES:
patent: 3579495 (1971-05-01), Huber
patent: 3624251 (1971-11-01), Huber
patent: 3687927 (1972-08-01), Huber
patent: 3763137 (1973-10-01), Huber et al.
patent: 3813289 (1974-05-01), Huber et al.
patent: 3832338 (1974-08-01), Huber et al.
McCord et al., Journal of Biological Chemistry, vol. 244, pp. 6049-6055, (1969).

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