Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Tissue – other than lymphoid tissue
Reexamination Certificate
2007-01-16
2007-01-16
Richter, Johann (Department: 1616)
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Tissue, other than lymphoid tissue
C424S520000, C424S522000, C424S537000, C424S543000, C424S548000, C514S025000, C514S026000, C514S033000, C514S034000, C514S885000
Reexamination Certificate
active
11145580
ABSTRACT:
This present invention discloses use of a chloroform/solvent mixture extraction at different solvent-to-feed ratios, followed by evaporation and extraction with ethyl acetate/water; followed by chromatography of the water phase in Teflon or other non-polar resin and Silica gel column chromatography for recovering of individual triterpene glycosides (saponins) of high purity from the freeze dried or spray dried cooking water or dried powderized tissues of the industrial processed sea cucumberCucumaria frondosa. A resulting glycoside Frondoside A stimulates lysosomal activity of peritoneal macrophages, phagocytosis and oxidative burst in the macrophages at concentrations significantly less than for acute toxicity, hemolysis and sea urchin embryo toxicity.
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Agafonova Irina Grigorevna
Aminin Dmitry Lvovich
Avilov Sergey Anatolievuch
Collin Peter D.
Kalinin Vladimir Ivanovich
Arnold Ernst V
Gugliotta John D.
Richter Johann
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