Process for isolating nucleic acids utilizing protease digestion

Details Process for isolating nucleic acids utilizing protease digestion Process for isolating nucleic acids utilizing protease digestion

1990-05-09

1991-11-05

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435262, 536 27, 536 28, 536 29, C12S 320, C12N 950, C12N 1512

Patent

active

050631624

ABSTRACT:
A process for the isolation of nucleic acids from cell or tissue extracts by precipitating the nucleic acids with a water-soluble ketone, preferably with acetone, optionally in the presence of dimethylformamide or formamide.

REFERENCES:
patent: 3582468 (1971-06-01), Birnbaum et al.
patent: 3597318 (1971-08-01), Sutherland et al.
patent: 3616211 (1971-10-01), Pietsch
patent: 3770584 (1973-11-01), Shaltiel et al.
patent: 4007265 (1977-02-01), Helting
patent: 4843012 (1989-06-01), De Bonville et al.
patent: 4865983 (1989-09-01), Durham
patent: 4868120 (1989-09-01), Eguchi et al.
patent: 4885236 (1989-12-01), Penman
patent: 4900677 (1990-02-01), Hewitt
"Biochemical and Organic Compounds for Research", Sigma Chemical Company, St. Louis, Mo., 1984, p. 767.
Kupiec et al., Anal. Biochem., vol. 164, 53-59 (1987).
Spencer et al., Biochim. Biophys. Acta, vol. 390, pp. 141-154 (1975).
Kochetkov et al., Org. Chem. Nucleic Acids, Part A, pp. 18-28 (1971).
Aonuma et al., Biol. Abst., 70(2):11700 (1979).
Hellen et al., Biol. Abst., 85(5):53654 (1988).
Imai, Chem. Abst., 106:98488v (1987).
Clark et al., Exp. Biochem., pp. 73-74 (1977).
Scopes, Protein Purification, pp. 52-57 (1982).

Affiliated with

Also associated with

Rating

Process for isolating nucleic acids utilizing protease digestion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for isolating nucleic acids utilizing protease digestion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for isolating nucleic acids utilizing protease digestion will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-498819