Process for isolating nucleic acid

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 422 61, 536 254, C12Q 168

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052348091

ABSTRACT:
The invention relates to a process, a combination of means for isolating nucleic acid from a nucleic acid-containing starting material and a testkit in order to amplify the nucleic acid obtained by said process. More in particular, the invention relates to a process and a kit for isolating nucleic acid from a nucleic acid-containing biological material such as whole blood, blood serum, urine, feces, cell cultures and the like.

REFERENCES:
patent: 4483920 (1984-11-01), Gillespie et al.
Cox et al. A Single step procedure for the isolation of individual mRNA species from crude lysates of P. polycephalum, FEBS Letters, vol. 155, #1, May 1983.
Krawety et al. Isolation & Fractionation of total nucleic acids from tissues & cells, J. of Biochem. Biophys. Methods 1986 12/1-2 (29-36) (abstract only).
M. A. Marko et al., "A Procedure for the large-scale isolation of highly purified plasmid DNA using Alkaline extraction and binding to glass powder", Anal. Bio., 121, pp. 382-387 (1987), USA.
Rikaken, K. K., "Glass Particles for recovery of DNA", Chemical Abstracts, vol. 102, 1985, No. 200711-u, USA.
J. Xuan et al., "Recovery of DNA for agarose gel using glass powder adsorption method and its application to the isolation of yeast plasmids" Chemical Abstracts, vol. 101, No. 187466a, 1984, USA.

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