Process for isolating human globular domain NC1 of basal membran

Details Process for isolating human globular domain NC1 of basal membran Process for isolating human globular domain NC1 of basal membran

1984-10-25

1989-01-17

Tarcza, John

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435 69, 530356, 530416, 530848, 530835, 530849, C07K 302, C07K 322, C07K 1520

Patent

active

047988002

ABSTRACT:
The present invention provides human characterized by a molecular weight of about 170,000 Dalton, a hexameric structure with monomeric subunits with a molecular weight of about 28,000 Dalton and a carbohydrate content of about 2%.
The present invention provides a process for obtaining and purifying globular domain NC1 of basal membrane collagen, wherein human or animal tissue is subjected to a first limiting treatment with bacterial collagenase, the degradation products obtained are separated from non-collagen proteins by chromatography on a weakly basic anion exchanger, the collagen degradation products are then subjected to a second collagenase digestion at an elevated temperature and the globular domain NC1 is purified by molecular sieve fractionation.
Furthermore, the present invention is concerned with the use of this for the determination in body fluids in the case of the use of their antibodies, as well as far the detection of antibodies directed thereagainst in body fluids.

REFERENCES:
patent: 4340581 (1982-07-01), Timpl
Timpl et al, "A Network Model for Organization of Type IV Collagen Molecu in Basement Membranes" European Journal of Biochemistry 120 pp. 203-211 (1981).
Weber et al, "Subunit Structure and Assembly of the Globular Domain of Basement Membrane Collagen Type IV", Eur J Biochem, vol. 139:401-410 (1984)(Mar. 1st).
Risteli et al, "7-S Collagen: Characterization of an Unusual Basement Membrane Structure", Eur J. Biochem, vol. 108:239-250(1980).

Affiliated with

Also associated with

Rating

Process for isolating human globular domain NC1 of basal membran does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for isolating human globular domain NC1 of basal membran, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for isolating human globular domain NC1 of basal membran will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2413463