Process for isolating human albumin from supernatant IV, in part

Details Process for isolating human albumin from supernatant IV, in part Process for isolating human albumin from supernatant IV, in part

1992-02-06

1994-09-13

Wityshyn, Michael G.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

530362, 530363, 530380, 530416, 530417, 210263, 210290, 210656, 210660, 210666, C07K 300, B01D 1500

Patent

active

053469925

ABSTRACT:
The process for isolating albumin from supernatant IV, and in particular IV-4, or from COHN's fraction V or from a plasma supernatant or fraction of analogous composition derived from an alcoholic or nonalcoholic fractionation, comprises one step on a hydrophilic anion exchange column with binding of albumin to the column, and then elution, and one step on a hydrophobic anion exchange column. Albumin thus obtained having a purity >99% by cellulose acetate electrophoresis, being free from impurities detected by crossed immunoelectrophoresis and free from polymers.

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