Process for isolating galactose oxidase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435911, C12N 904

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active

051496461

ABSTRACT:
A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a carboxy-sulfon cation exchange column using high performance liquid chromatography. Copper ions must be present to insure enzyme activity.

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Israel J. Chem., 3, 193 (1966).

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