Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Patent
1999-02-25
2000-03-07
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
4242041, 935 57, C12N 702, C12N 701, A61K 3912
Patent
active
060338954
ABSTRACT:
The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.
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Garger Stephen J.
Holtz R. Barry
McCulloch Michael J.
Turpen Thomas H.
Biosource Technologies, Inc.
Gallegos, Esq. Thomas
Gitomer Ralph
Halluin, Esq. Albert P.
Moran Marjorie A.
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