Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1998-03-10
2000-03-14
Gitomer, Ralph
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530379, 435419, 435239, 4241951, 536128, C07K 114, C07K 136, A61K 3578, C07H 108, C12N 702
Patent
active
06037456&
ABSTRACT:
The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspenion, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.
REFERENCES:
patent: 3637396 (1972-01-01), Hollo et al.
patent: 4130553 (1978-12-01), Batley, Jr. et al.
patent: 4233210 (1980-11-01), Koch
patent: 4250197 (1981-02-01), Koch
patent: 4268632 (1981-05-01), Wildman et al.
patent: 4289147 (1981-09-01), Wildman et al.
patent: 4334024 (1982-06-01), Johal
patent: 4347324 (1982-08-01), Wildman et al.
patent: 4370267 (1983-01-01), Lehnhardt et al.
patent: 4396763 (1983-08-01), Tsuchiya et al.
patent: 4400471 (1983-08-01), Johal
patent: 4885248 (1989-12-01), Ahlquist
patent: 5077390 (1991-12-01), Wu et al.
patent: 5173410 (1992-12-01), Ahlquist
patent: 5301694 (1994-04-01), Raymond et al.
patent: 5316931 (1994-05-01), Donson et al.
patent: 5320953 (1994-06-01), Hudson et al.
patent: 5466788 (1995-11-01), Ahlquist et al.
patent: 5500360 (1996-03-01), Ahlquist et al.
patent: 5589367 (1996-12-01), Donson et al.
patent: 5602242 (1997-02-01), Ahlquist et al.
patent: 5627060 (1997-05-01), Ahlquist et al.
patent: 5811654 (1998-09-01), Jaynes et al.
Brakke, M., "Density Gradient Centrifugation and its Application to Plant Viruses", Adv. Virus. Res. 7:193-224 (1960) no month found.
Gooding et al., "A Simple Technique for Purification of Tobacco Mosaic Virus in Large Quantities", Phytopathological Notes 57:1285 (Nov. 1967).
Khan et al., "Accumulation of a sulphur-rich seed albumin from sunflower in the leaves of transgenic subterranean clover (Trifolium subterraneum L.)", Transgenic Res. 5:178-185 (1996) no month found.
Mason et al., "Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice", Proc. Natl. Acad. Sci. USA 93:5335-5340 (May 1996).
Turpen et al., "Malarial Epitopes Expressed on the Surface of Recombinant Tobacco Mosaic Virus", BioTechnology 13:53-57 (Jan. 1995).
Ma et al., Science 268:716-719 (May 1995).
Garger Stephen J.
Holtz R. Barry
McCulloch Michael J.
Turpen Thomas H.
Biosource Technologies, Inc.
Gallegos, Esq. Thomas
Gitomer Ralph
Halluin, Esq. Albert P.
Moran Marjorie A.
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