Compositions – Nonreactive analytical – testing – or indicating compositions
Patent
1976-12-27
1979-03-27
Padgett, Benjamin R.
Compositions
Nonreactive analytical, testing, or indicating compositions
23230B, 195 17, 1951035R, 424 1, 424 10, 424 95, 424104, C09K 300, G01N 100, G01N 3114, G01N 3316
Patent
active
041465016
ABSTRACT:
A process for isolating and purifying taste bud receptor material for screening and evaluating tastants is described. The process includes surgically excising a plurality of circumvallate papillae, coring said papillae to remove the central mucous gland from each, followed by exposing the taste bud bearing epithelial portion thereof. The buds are preferably exposed by swelling in a hypotonic medium followed by subjecting said buds to about 900 psi in a nitrogen bomb. The swelled buds are then subjected to a gentle, selective homogenization to extrude taste bud receptor material therefrom without substantial destruction of surrounding epithelial tissue. The extruded material is then isolated by filtering and purified by subjecting the filtrate to differential centrifugation to isolate a receptor containing membrane enriched fraction. The fraction is then further purified by sucrose gradient centrifugation to isolate a receptor containing membrane enriched subfraction substantially without contaminating materials capable of binding tastants. The subfraction is then utilized as a medium for evaluating tastants wherein radioactive labeled tastant compounds are incubated with said subfraction until equilibrium is reached, and the quantity of labeled tastant is bound to the receptor material may then be measured. Unlabeled tastants may also be incubated with said labeled tastant bound medium to evaluate the strength of the binding, and other related taste characteristics.
REFERENCES:
lum, Clark., K. L. et al., Fed. Proc., Vol. 32, No. 3, 328 Abs., 634 (Mar. 1973).
Lo, Chai-Ho; Biochim. Biophys. Acta, vol. 291, pp. 650-661 (1973).
Cagan, R. H., Biochim, Biophys. Acta., vol. 252, pp. 199-206 (1971).
Touster, O., et al., J. Cell. Biol., vol. 47, pp. 604-618 (1970).
Lum, Clark K. L., et al., Biochim. Biophys. Acta, vol. 421, pp. 353-361, pp. 362-379, pp. 380-394 (Feb. 1976).
Gron, T.
Padgett Benjamin R.
LandOfFree
Process for isolating and purifying taste bud receptors for scre does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for isolating and purifying taste bud receptors for scre, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for isolating and purifying taste bud receptors for scre will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1821102