Process for isolating and purifying nucleotide-activated sugars

Details Process for isolating and purifying nucleotide-activated sugars Process for isolating and purifying nucleotide-activated sugars

1995-08-30

1998-09-22

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 172, 536 179, 536 265, 536 285, 536 553, C07H 1906, C07H 1916

Patent

active

058115390

ABSTRACT:
The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleotides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromatographic method which uses silica gel as the stationary phase, and at a purity level which requires only a subsequent desalting step to obtain a substantially pure product.

REFERENCES:
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