Process for isolating and preparing purified chitin deacetylase

Details Process for isolating and preparing purified chitin deacetylase Process for isolating and preparing purified chitin deacetylase

1991-10-09

1993-06-15

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 711, 435 84, 435178, 435259, 435931, 536 20, C12N 978, C12R 1785, C12P 1926

Patent

active

052197492

ABSTRACT:
Chitin deacetylase, the enzyme that catalyzes the hydrolysis of acetamide groups of N-acetylglucosamine in chitin, was purified to homogeneity from mycelial extracts of the fungus Mucor rouxii. In addition, immunoglobulin specifically reactive with chitin deacetylase has been produced and purified.

REFERENCES:
patent: 4282351 (1981-08-01), Muzzarelli
patent: 4368322 (1983-01-01), Muzzarelli
patent: 4958011 (1990-09-01), Bade
Pfaltz & Bauer Catalogue 1991/1992. 12th Ed. Div Aceto Chem Waterbury, Conn. 06708 p. 81 NBR C07640 "Chitin Deacetylated".
Master's Thesis of Stephen J. Lombardi.
Araki and Ito, Methods in Enzymol., 161: 510-514 (1988).
Kauss and Bauch, Methods in Enzymol., 161: 518-523 (1988).

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