Process for isolating active debriding agent from bromelain

Details Process for isolating active debriding agent from bromelain Process for isolating active debriding agent from bromelain

1979-11-05

1981-08-25

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435816, C12N 950, C12N 948

Patent

active

042860644

ABSTRACT:
A process is disclosed for isolating an enzyme mixture useful in the treatment of devitalized tissue from crude bromelain. The process comprises the steps of: (1) suspending the crude bromelain in a weakly basic buffer, preferably sodium borate buffer, to selectively dissolve the active enzyme mixture; (2) separating the undissolved solids from the solution; and (3) removing small molecules having a molecular weight of 10,000 or less from the solution.

REFERENCES:
patent: 3442764 (1969-05-01), Young et al.
patent: 3446626 (1969-05-01), McAnelly et al.
patent: 3446706 (1969-05-01), Beuk
patent: 4197291 (1980-04-01), Klein
Silverstein et al., "In Vitro Evaluation of Enzymatic Debridement of Burn Wound Eschar", Surgery, vol. 73, No. 1, pp. 15-22, (Jan. 1973).
Levine et al., "Debridement of Experimental Skin Burns of Pigs with Bromelain, a Pineapple Stem Enzyme", Plastic and Reconstr. Surg., vol. 52, No. 4, pp. 413-424 (Oct. 1973).
Levenson et al., "Chemical Debridement of Burns", Ann. Surg., vol. 180, No. 4, pp. 670-704 (1974).

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