Process for isolating a fibrin-stabilizing factor

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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A61K 3548

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active

039313999

ABSTRACT:
A process for isolating a fibrin-stabilizing factor, factor XIII, by extracting human placentae with sodium chloride solution; adding diamino-ethoxy-acridine lactate (DEAL); recovering the resultant precipitate and dissolving it in dilute alkali metal chloride solution containing a complexing agent; adding a quaternary ammonium base to the solution to precipitate inactive materials; reprecipitating the active principal from solution with DEAL; again dissolving the precipitate in dilute alkali metal chloride solution containing a complexing agent; reprecipitating the active principal by adding solid ammonium sulfate; forming a paste between the precipitate and a dilute solution of a complexing agent; dialyzing the paste; gel filtering the dialyzed product; precipitating the active principal from the active fractions with ammonium sulfate; dissolving the precipitate in a neutral tris-(hydroxymethyl)-aminomethane hydrochoric acid buffer containing a complexing agent; dialyzing the solution; and precipitating the factor XIII from the remaining solution in the form of euglobulin.

REFERENCES:
patent: 3497492 (1970-02-01), Buck et al.

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