Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Patent
1989-08-29
1991-12-10
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
4351723, 4353221, 435256, 236 27, 935 28, 935 29, 935 64, 935 75, 935 76, C12N 1500, C12N 116, C12P 1931
Patent
active
050717642
ABSTRACT:
Process for transformation of Yarrowia lipolytica, vectors useful therefor comprising DNA of a microbial vector and chromosomal DNA of Y. lipolytica and transformants comprising said vectors in E. coli and Y. lipolytica, and integrative shuttle vectors for Escherichia-Yarrowia transgeneric cloning. Said vectors or subclones thereof enable creation of Y. lipolytica cloning vectors into which specific or random segments of DNA can be inserted and the resulting vectors used to transform a suitable host microbe, especialy Y. lipolytica, to improve the fermentation characteristics thereof and hence their industrial utilization.
The methodology described permits the cloning of genes from a gene library of Y. lipolytica by complementation with an integrating vector.
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Davidow Lance S.
DeZeeuw John R.
Benson Gregg C.
Lumb J. Trevor
Pfizer Inc.
Richardson Peter C.
Teskin Robin L.
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