Process for integration of a chosen gene on the chromosome of a

Details Process for integration of a chosen gene on the chromosome of a Process for integration of a chosen gene on the chromosome of a

1994-11-17

1997-01-21

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 691, 435 711, 4351723, 435243, 4352523, 435847, 435849, 935 42, 935 52, 935 72, 935 73, C12N 100, C12N 1500, C12P 2102, C12P 100

Patent

active

055958893

ABSTRACT:
The present invention relates to a process for integration of a chosen gene or of a specific DNA molecule into the chromosome or episome of a bacterium by cloning the gene or DNA molecule into a defective transposon which is then integrated into the chromosomal or episomal DNA of the bacteria. The defective transposon is incapable of transposition autonomously but can be induced to transpose when properly complemented. The complementation to induce transposition can be limited so that the defective transposon produces a specific number of copies and thereafter is stable. The number of copies of the defective transposon produced during the period of transposition can be estimated by the level of expression of a marker gene contained within the defective transposon.

REFERENCES:
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