Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1984-08-09
1986-11-11
Marantz, Sidney
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435173, 435240, 935 93, 204131, 204DIG8, 204DIG9, C12N 1300, C12N 500, C12N 1500
Patent
active
046223029
ABSTRACT:
A device and process for inducing membrane fusion under an electric field. The process comprises the steps of (a) suspending said membranes in an aqueous buffered medium in a manner so that the membranes are without contact with each other; (b) altering said membranes to a fusogenic state by applying 2-30 pulses of direct current at a rate of 2 to 5 pulses per second in an electrical field strength of about 500-1000 volts/mm in the medium, said pulses having a rapid rise time not exceeding about 10 micro seconds and an exponential decay half time of about 0.2 milliseconds to about 1.2 milliseconds; and (c) thereafter bringing said fusogenic membranes in contact with each other. A long-lived metastable fusogenic state of membranes can be obtained by the device and process.
REFERENCES:
patent: 2440472 (1948-04-01), Horner et al.
patent: 2955076 (1960-10-01), Gossling
patent: 3095359 (1963-06-01), Heller
patent: 4292408 (1981-09-01), Zimmermann et al.
patent: 4561961 (1985-12-01), Hofman
patent: 4578168 (1986-03-01), Hofman
Benz et al., The Resealing Process of Lipid . . . , Biochimica et Biophysica Acta, 640; 1981; pp. 169-178.
Zimmermann et al., Electric Field-Induced Cell-to-Cell Fusion; J. Membrane Biol. 67; pp. 165-182 (1982).
Zimmermann et al., Electric Field Induced Cell Fusion; BioTechniques; Sep.-Oct. 1983; pp. 118-122.
Crane; American Biotechnology Laboratory 1, An Electro Cell Fuser; pp. 74-79; 1983.
Zimmermann et al., Electric Field-Mediated Cell Fusion; J. Biol. Phys., vol. 10, 1982; pp. 43-50.
Knutton et al., The Mechanism of Cell-Cell Fusion; TIBS-Oct. 1979; pp. 220-223.
Henderson et al., Three-Dimensional Model of Purple Membrane Obtained by Electron Microscopy; Nature, vol. 257, Sep. 1975; pp. 28-32.
Lucy, "Mechanisms of Chemically Induced Cell Fusion" in Poste et al. (ed.), Membrane Fusion, Elsevier/North-Holland Biomedical Press, 1978, pp. 267-304.
Zimmermann, Biochimica et Biophysica Acta, 694, (1982), pp. 227-277.
Neumann et al., The EMBO Journal, vol. 1, No. 7, 1982, pp. 841-845.
American National Red Cross
Deck Randall E.
Marantz Sidney
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