Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Reexamination Certificate
2006-02-07
2006-02-07
Kubelik, Anne (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
C435S430100, C800S278000
Reexamination Certificate
active
06995016
ABSTRACT:
A process is provided for inducing direct somatic embryogenesis in Pooideae and rapidly regenerating fertile plants by first culturing isolated immature scutella cells in culture medium comprising auxin, cytokinin and polyamine in amounts effective to cause direct formation of primary embryos without an intervening callus stage, at least until at least one primary embryo reaches the globular developmental stage, the auxin being present in greater proportion than cytokinin. A second step includes either a) culturing the primary embryos under conditions to regenerate plantlets, and culturing the primary embryos in regeneration medium; or b) culturing the primary embryos at the globular developmental stage and no longer than the coleoptilar stage in culture medium comprising auxin, cytokinin, and polyamine in amounts effective to cause induction of secondary embryo formation, at least until secondary embryogenesis is detected, the cytokinin being present in greater proportion than auxin, and culturing the secondary embryos under conditions to regenerate plantlets.
REFERENCES:
patent: 4666844 (1987-05-01), Cheng
patent: 5322789 (1994-06-01), Genovesi et al.
patent: 5409828 (1995-04-01), Frenkel et al.
patent: 5445961 (1995-08-01), Genovesi et al.
patent: 5589617 (1996-12-01), Nehra et al.
patent: 5610042 (1997-03-01), Chang et al.
patent: 5631152 (1997-05-01), Fry et al.
patent: 5641664 (1997-06-01), D'Halluin
patent: 5712135 (1998-01-01), D'Halluin
patent: 5792936 (1998-08-01), Dudits et al.
patent: 5874265 (1999-02-01), Adams et al.
patent: 5981842 (1999-11-01), Wu et al.
patent: 6071746 (2000-06-01), Seabrook et al.
patent: 1292959 (1991-10-01), None
patent: 0720 425 (1998-02-01), None
patent: WO 99/04618 (1999-02-01), None
patent: WO 00/16610 (2000-03-01), None
Denchev et al. Transgenic orchardgrass (Dactylis glomerata) plants by direct embryogenesis from microprojecticle bombarded leaf cells. 1997. Plant Cell Reports, 16: 813-819.
Mariani et al. Changes in Surface Structure during Direct Somatic Embryogenesis in Rice Scutellum Observed by Scanning Electron Microscopy. 1998. Plant Prod. Sci. 1 (3) : 223-231.
Jong et al. Somatic Embryogenesis and Plant Regeneration from Inflorescence Callus ofZizania latifoliaTurcz. 1987. J. Plan Physiol. vol. 130, pp. 67-71.
Sankhla et al. Influence of growth regulators on somatic embryogenesis, plantlet regeneration, and post-transplant survival ofEchinochloa frumentacea. 1992. Plant Cell Reports 11: 368-371.
Sudhersan et al. Occurrence of direct somatic embryogenesis on the sword leaf of in vitro plantlets of Phoenix dactylifera L. cultivar barhee. 1993. Current Science, vol. 65, No. 11, pp. 887-889.
Dale. Embryoids from Cultured Immature Embryos ofLolium multiflorum. Z. Pflanzenphysiol. Bd. 100. S. 73-77. 1980.
Dunstan et al. Further Studies on Plantlet Production From Cultured Tissues ofSorghum bicolor.Protoplasma 101, pp. 355-361, 1979.
Dunstan et al. The Anatomy of Secondary Morphogenesis in Cultured Scutellum Tissues ofSorghum bicolor.Protoplasma 97 pp. 251-260, 1978.
Mantell et al. Principles of Plant Biotechnology an Introduction to Genetic Engineering in Plants. Blackwell Scientific Publications, pp. 141-143, 1985.
Sargent et al. The effect of exogenous polyamines on somatic embryogenesis and plant regeneration fromSorghum bicolorandSaccharum spp.Acta horticulturae, Aug. 1998. No. 461, pp. 451-458.
Ahokas, H. (1989) Transfection of germinating barley seed electrophoretically with exogenous DNA. Theor. Appl. Genet. 77:469-472.
Ahloowalia, B.S. (1982) Plant regeneration from callus culture in wheat. Crop Sci. 22: 405-410.
Alber, T. and Kawasaki, G. (1982) Nucleotide sequence of the triose phosphate isomerase gene ofSaccharomyces cerevisiae. Journal of Molecular and Applied Genetics 1: 419-434.
Antonelli, N.M. and Stadler, J. (1990) Genomic DNA can be used with cationic methods for highly efficient transformation of maize protoplasts. Theor. Appl. Genet. 80: 395-401.
Arteca, R. (1996) Plant Growth Substances: Principles and Applications. New York. Chapman & Hall.
Barcelo, P., Hagel C., Becker, D., Martin, A. and Lörz H. (1994) Transgenic cereal (tritordeum) plants obtained at high efficiency by microprojectile bombardment of inflorescene tissue. Plant Journal 5: 583-592.
Barro, F., Cannell, M.E., Lazzeri, P.A. and Barcelo, P. (1998) The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants. Theor. Appl. Genet. 97: 684-695.
Becker, D., Brettschneider, R. and Lörz, H. (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant Journal 5:299-307.
Bevan, M.W., Flavell, R.B. and Chilton, M.D. (1983) A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184-187.
Bhaskaran, S. and Smith, R.H. (1990) Regeneration in cereal tissue culture: a review. Crop Sci. 30(6):1328-1337.
Bregitzer, P., Dahleen, L.S. and Campbell, R.D. (1998) Enhancement of plant regeneration from embryogenic callus of commerical barley cultivars. Plant Cell Rep. 17: 941-945.
Burkhardt, P.K., Beyer, P., Wunn, J., Kloti, A., Armstrong, G.A., Schledz, M., Lintig, J. and Potrykus, I. (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key Intermediate of provitamin A biosynthesis. Plant J. 11: 1071-1078.
Carman, J.G. (1990) Embryogenic cells in plant tissue cultures: occurrence and behavior. In Vitro Cell Dev. Biol.:26: 746-753.
Cho, M.J., Jiang, W.I. and Lemaux, P. (1998) Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism. Plant Science 138: 229-244.
Creissen, G., Smith, C., Francis, R., Reynolds, H. and Mullineaux, P. (1990)Agrobacterium—and microprojectile—mediated viral DNA into barley microspore derived cultures. Plant Cell Rep. 8: 680-683.
De La Fuente, J.M., Ramirez-Rodriguez, V., Cabrera-Ponce, J.L. and Herrera-Estrella. L. (1997) Aluminum tolerance in transgenic plants by alteration of citrate synthesis. Science 276: 1566-1568.
Depicker, A., Strachel, S., Dhaese, P., Zambryski, P. and Goodman, H.M. (1982) Nopaline synthase: transcript mapping and DNA sequenceAgrobacterium tumelaciens. J. Mol. Appl. Genet. 1(6): 561-573.
Elliott, M.C., Moloney, M.M., Scott, N.W. and Slater, A. (1996) A way forward for tobacco—molecular farming. Biotech. and Biotechnol. Equip. 4: 59-66, (Abstract only).
Fischer, R., Drossard, J., Commandeur, U., Schillberg, S. and Emans, N. (1999) Towards molecular farming in the future: moving from diagnostic protein and antibody production in microbes to plants. Biotech. and Apol. Biochem. 30(2): 101-108.
Fromm, M.E., Taylor, L.P. and Walbot, V. (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319: 791-793.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151-158.
George, E.F., Puttock, D.J.M. and George, H.L. (1987) Plant Culture Media vol. 1, Formulations and Uses. Exegetics Limited.
Gielen, J., De Beuckeleer, M., Seurinck, J., Deboeck, F. and De Greve, H. (1984) The complete nucleotide sequence of the TL-DNA of theAgrobacterium tumefaciensplasmid pTiAch5. EMBO J. Eur. Mol. Biol. Organ. 3(4):835-846.
Green C.E. (1982) Somatic embryogenesis and plant regeneration from the friable callus ofZea mays. In Plant Tissue Culture 1982: Proceedings, Fifth International Congress of Plant Tissue and Cell Culture. Fujiwara, A. Ed. Tokyo and Lake Yamanake, Japan, Jul. 11-16, pp. 107-108.
Gruber, Margaret Y. Crosby, William L. (1993) Vectors for Plant Transformation. Methods in Plant Molecular Biology and Biotechnology. Boca Raton, CRC Press. Chapter 7:89-119.
Hayashi, H., Alia., Mustardy, L., Deshnium, P., Ida, M. and Murata. N. (1997) Transformation ofArabidopsis thalianawith the codA gene for choline oxidase; accumlation of glycinebetaine and enhanced tolerance to salt and cold stress. Plant J. 12: 133-142.
Hemmi
Acharya Surya Narayan
Eudes Francois Andre Germain
Laroche Andre J.
Greenlee Winner and Sullivan P.C.
Her Majesty the Queen in right of Canada as represented by the
Hwu June
Kubelik Anne
LandOfFree
Process for inducing direct somatic embryogenesis in... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for inducing direct somatic embryogenesis in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for inducing direct somatic embryogenesis in... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3697072