Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-08-12
1999-02-02
Witz, Jean C.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
43525421, 4352931, 530427, C12N 950, C12N 114, A23J 100, C12M 132
Patent
active
058663991
ABSTRACT:
The present invention relates to a process for inactivating Carboxypeptidase Y (CPY) in a hirudin-containing culture broth produced by fermenting a transformed yeast. It has been found that inactive precursors of CPY are activated at a temperature 70.degree. C. and that the active peptidases will further degrade hirudin when the two proteins are present together in a composition. The present invention overcomes the CPY activation problem by heating a broth containing hirudin and CPY to a temperature of about 80.degree.-100.degree. C. in about one minute or less.
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Crause Peter
Habermann Paul
Moller Jorg
Ulmer Wolfgang
Hanley Susan
Hoechst Aktiengesellschaft
Witz Jean C.
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