Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-05-10
1998-01-27
Zitomer, Stephanie W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 435 792, 530334, 530344, 935 77, 935 78, C07H 2104, C07H 2102, G01N 3353, C12Q 168
Patent
active
057123837
ABSTRACT:
Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.
REFERENCES:
patent: 4657853 (1987-04-01), Freytag et al.
patent: 4657873 (1987-04-01), Gadow et al.
patent: 4868105 (1989-09-01), Urdea et al.
Nakagami et al., "Preparation of enzyme-conjugated DNA probe and application to the universal probe system" Anal. Biochem. (1991) 198:75-79 .
Chang Chu-An
Running Joyce
Sheridan Patrick
Urdea Michael S.
Blackburn Robert P.
Chiron Corporation
Dylan, Ph.D. Tyler
Goldman Kenneth M.
Zitomer Stephanie W.
LandOfFree
Process for immobilizing nucleic acid probes on polystyrene surf does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for immobilizing nucleic acid probes on polystyrene surf, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for immobilizing nucleic acid probes on polystyrene surf will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-343473