Process for immobilizing a nucleic acid fragment by passive atta

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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435 6, 436 94, G01N 3348, B01L 900

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active

055100848

ABSTRACT:
In a process for immobilization by passive fixation on a solid support of a nucleic acid fragment containing less than 100 nucleotides, the fragment is deposited on the support in the form of a derivative. The derivative results from the covalent coupling of the fragment with a ligand having a molecular mass of less than 5,000 and containing at least one polar functional group. The derivative is not capable of forming a covalent bond with the support under the conditions of the deposition. When the ligand is a nucleotide or an oligonucleotide it is at least one nucleotide modified so as to introduce the polar functional group.

REFERENCES:
patent: 4379843 (1983-04-01), Cashion
patent: 4739044 (1988-04-01), Stabinsky
patent: 4828979 (1989-05-01), Klevan et al.
patent: 4948882 (1990-08-01), Ruth
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 5112734 (1992-05-01), Kramer et al.
patent: 5138045 (1992-08-01), Cook et al.
patent: 5185439 (1993-02-01), Arnold, Jr. et al.
patent: 5232829 (1993-08-01), Longiaru et al.
M. Zouali et al, "A Rapid ELISA for Measurement of Antibodies to Nucleic Acid Antigens Using UV-treated Polystyrene Plates", Journal of Immunological Methods, vol. 90, 1986, pp. 105-110.
R. L. Rubin et al, "An Improved ELISA for Anti-Native DNA by Elimination of Interference by Anti-Histone Antibodies", Journal of Immunoligical Methods, vol. 63, No. 3, 1983, pp. 359-366.
Dunn et al, "A Novel Method to Map Transcripts: Evidence for Homology Between An Adenovirus mRNA and Discrete Multiple Regions of the Viral Genome", Cell, vol. 12, 23-36, Sep., 1992.
Saiki et al, "Genetic Analysis of Amplified DNA with Immobilized Sequence-Specific Oligonucleotide Probes", Proc. Natl. Acad. Sci. USA, vol. 86, pp. 6230-6234, Aug., 1989.
Ranki et al, "Sandwich Hybridization As A Convenient Method for the Detection of Nucleic Acids in Crude Samples", Gene, 21, 1983, pp. 77-85.
Palva et al, "Detection of Chlamydia Trachomatis by Nucleic Acid Sandwich Hybridization", FEMS Microbiology Letters, 23, 1984, pp. 83-89.
Polsky-Cynkin et al, "Use of DNA Immobilized on Plastic and Agarose Supports to Detect DNA by Sandwich Hybridization", Clinical Chemistry, vol. 31, No. 9, 1985, pp. 1438-1443.
Kremsky et al., Nucleic Acids Res. 15(7), 2891-2909 (1987).
Zendegui et al., Nucleic Acids Res., 20(2), 307-314 (1992).

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