Process for immobilization of glucoamylase

Details Process for immobilization of glucoamylase Process for immobilization of glucoamylase

1986-07-29

1988-12-27

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435181, 435205, C12N 1108, C12N 1106, C12N 934

Patent

active

047940832

ABSTRACT:
Glucoamylase is immobilized with a carbodiimide on a polymer having amino or carboxy functional groups. When the polymer contains amino groups, the glucoamylase is treated with the carbodiimide and then mixed with the polymer or the carbodiimide is added to a mixture of the glucoamylase and the polymer. When the polymer contains carboxy groups, the polymer is treated with the carbodimide and then mixed with the glucomylase. The polymer is produced from acrylic acid, metharylic acid, acrylamide, methacrylamide, N-amino-ethyl-acrylamide and/or N-amino ethyl methacrylamide and a cross-linking agent of the acrylic or allylic type.

REFERENCES:
patent: 3860490 (1975-01-01), Guttag
patent: 3871964 (1975-03-01), Huper et al.
patent: 4349630 (1982-09-01), Maximenko et al.
patent: 4352884 (1982-10-01), Nakashima et al.
patent: 4371612 (1983-02-01), Matsumoto et al.
Immobilized Enzymes, Research and Development, ed. by Ichiro Chibata, Kodansha Scientific Books, Japan, 1978, pp. 23, 27, 31, 32.

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