Process for identifying nucleic acids and triple helices formed

Details Process for identifying nucleic acids and triple helices formed Process for identifying nucleic acids and triple helices formed

1993-11-12

1998-08-04

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 935 77, 935 78, C12Q 168, C07H 2104

Patent

active

057891559

ABSTRACT:
Probes and processes for their use for specific recognition and/or cleavage of double-stranded DNA or RNA at sequence specific desired loci through the intermediacy of a triple helix are disclosed. These probes may also be used as diagnostic chemotherapeutic agents through incorporation of a radiolabeled, fluorescing, or otherwise detectable molecule. Preferred assay conditions are also provided for recognition of homopurine-homopyrimidine double-helical tracts within large DNA by triple helix formation under physiological conditions. Hybridization probes for double-stranded recognition with binding site sizes that range >8 base pairs are also provided.

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